Fig. 6

Salt stress tolerance analyses of the CenREV3 Arabidopsis overexpression lines. Ai) Expression (FPKM) and Aii) CT (qRT-PCR) of CenREV3 in C. ensifolium leaves at CK, T48, and T96. The values are the mean ± SD of five replicates (p < 0.05). The asterisk sign (*) represents significant differences. B) Selection and confirmation of transgenic Arabidopsis lines. Bi) Transgenic lines selected on selection medium. Bii) Confirmation of positive transgenic Arabidopsis lines by PCR, where M = ladder, 2–11 wells are PCR confirmation results, and the last well represents vector control. Ci) Comparison of wild type and transgenic Arabidopsis seedlings under salt stress conditions (100 mM NaCl) for seven days. Cii) Morphological comparison of transgenic lines (TL) and wild-type after salt stress treatment (100 mM NaCl). Two rows present two different plants for the same line. C) Morphological analysis of the CenREV3 overexpression lines; Di) root length, Dii) germination %, and Diii) Survival %. E) Expression (CT (qRT-PCR) of CenREV3 in four transgenic lines. The qRT-PCR was repeated five times and the means were presented. Original pictures are presented in Supplementary data 1