Fig. 5From: Betalain biosynthesis in red pulp pitaya is regulated via HuMYB132: a R-R type MYB transcription factorTranscription effects of HuMYB132 on HuADH1, HuCYP76AD1–1, and HuDODA1 promoters by dual luciferase assay. A, Effector and reporter vector diagrams. B, HuMYB132 activated the transcription of HuADH1, HuCYP76AD1–1, and HuDODA1 promoters by dual-luciferase transient expression assay in N. benthamiana leaves. The LUC/REN ratio of the empty vector (pGreenII 62-SK) plus the HuADH1, HuCYP76AD1–1, and HuDODA1 promoters were used as a calibrator (set as 1). Small triangles represent the distribution of data for each biological replicate. Results were expressed as means ± SD (n = 3), with significance values presented as: *** p < 0.001; **** p < 0.0001Back to article page