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Fig. 2 | BMC Plant Biology

Fig. 2

From: Direct leaf-peeling method for areca protoplasts: a simple and efficient system for protoplast isolation and transformation in areca palm (Areca catechu)

Fig. 2

Effects of mannitol concentration on areca palm protoplast isolation. a Released protoplasts on a hemocytometer. Mannitol concentration was set at 0.2, 0.4, 0.6, 0.7, 0.8 and 1.0 M, respectively. Released protoplasts after enzyme solution digestion overnight were visualized under a light microscope. Scale bar = 100 μm; b Effect of mannitol concentration on areca protoplast yield. The number of round and intact protoplasts was counted under a light microscope. Error bars represent the SD of three replicates. Different lowercase letters represent significant statistical difference. c Viability test for protoplasts isolated with different mannitol concentrations. At least 80 protoplasts exhibiting green fluorescence under a fluorescent microscope were counted. Error bars represent the SD of four replicates. Different lowercase letters represent significant statistical difference

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BMC Plant Biology

ISSN: 1471-2229

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