Fig. 6

The expression patterns of CrLTPs transcripts and proteins in different developmental stages of Chlamydomonas. A RT-PCR analysis of the transcript levels of CrLTP1 and CrLTP2 in different developmental stages of Chlamydomonas, including the vegetative cells and gametes of both mating types, cc124 (mt⁻) and cc125 (mt⁺), and zygotes collected 6 hours, 12 hours, 1 day, 2 days and 3 days after mating. Actin was used as a normalization control. The displayed regions were cropped from three agarose gels loaded with the RT-PCR products using primers sets specific to CrLTP1, CrLTP2 and Actin. The full length gels are presented in Supplementary Fig. 5. B The expression pattern of CrLTP2 protein in Chlamydomonas. Total proteins of vegetative cells and gamete of both strains, and zygote cells collected from different time points after gamete mating were resolved with SDS-PAGE and visualized by immunoblotting against CrLTP2. Immunoblotting against α-tubulin was used as a quantity reference. A replicate gel stained with Coomassie brilliant blue was also provided as another reference of the loading control due to an unknown factor that abruptly weakened the signal of α-tubulin in the 4-d-old zygotes. The molecular masses are indicated at left in kDa. The displayed regions were cropped from the images captured from the same PVDF membrane blotted with CrLTP2 and α-tubulin antibodies. The full length blots and gel are presented in Supplementary Fig. 6