Fig. 3

Unrooted phylogenetic tree of nsLTPs from land plants and green algae. Protein sequences of the five major nsLTP types, including type I, type II, type C, type D, and type G, from the representative land plants along with the nsLTPs found in green algae were aligned with MAFFT program in default setting. The neighbor-joining tree was constructed using the programs in PHYLIP package. Only the bootstrap values above 50 are shown on the nodes. Each color represents different nsLTP types. The algae nsLTPs in the tree are KnLTP1, KnLTP2, APLTP1, APLTP2, CrLTP1, CrLTP2, CsLTP1, CsLTP2, CsLTP3, CsLTP4, CsLTP5, CsLTP6, CsLTP7, GpLTP, McLTP1, McLTP2, MnLTP1, MnLTP2, OtLTP1, OtLTP2, RsLTP1, RsLTP2, and TsLTP. Type I nsLTPs include AtLTPI.1, AtLTPI.2, AtLTPI.3, AtLTP1.4, AtLTP1.5, AtLTP1.6, AtLTP1.7, AtLTP1.8, AtLTP1.9, AtLTP1.10, AtLTP1.11, AtLTP1.12, OsLTP1.1, OsLTP1.2, OsLTP1.3, OsLTP1.4, OsLTP1.5, OsLTP1.6, OsLTP1.9, OsLTP1.12, OsLTP1.13, OsLTP1.14, OsLTP1.16, OsLTP1.17, OsLTP1.18, and OsLTP1.20. Type II nsLTPs include AtLTP2.2, AtLTP2.3, AtLTP2.4, AtLTP2.5, AtLTP2.6, AtLTP2.7, AtLTP2.9, AtLTP2.10, AtLTP2.11, AtLTP2.12, AtLTP2.13, AtLTP2.14, OsLTP2.1, OsLTP2.2, OsLTP2.3, OsLTP2.4, OsLTP2.5, OsLTP2.6, OsLTP2.7, OsLTP2.8, OsLTP2.10, OsLTP2.11, and OsLTP2.12. Type C nsLTPs include OsLTPc1, OsLTPc2, AtLTPc1, AtLTPc2, AtLTPc3, and AmboLTPc. Type D nsLTPs include AtLTPd1, AtLTPd3, AtLTPd4, AtLTPd5, AtLTPd6, AtLTPd7, AtLTPd8, AtLTPd9, AtLTPd10, AtLTPd12, OsLTPd1, OsLTPd2, OsLTPd3, OsLTPd4, OsLTPd5, OsLTPd6, OsLTPd7, OsLTPd8, OsLTPd9, OsLTPd10, OsLTPd12, PpLTPd1, PpLTPd3, PpLTPd4, PpLTPd5, PpLTPd7, PpLTPd8, PpLTPd10, PpLTPd11, PpLTPd12, PpLTPd13, PpLTPd14, PpLTPd15, PpLTPd16, PpLTPd17, PpLTPd18, PpLTPd19, PpLTPd20, PpLTPd22, SmLTPd3, SmLTPd4, SmLTPd5, SmLTPd6, SmLTPd7, SmLTPd9, SmLTPd10, SmLTPd12, SmLTPd13, SmLTPd16, MpLTPd1, MpLTPd2, MpLTPd3, MpLTPd4, MpLTPd5, MpLTPd6, MpLTPd7, MpLTPd8, MpLTPd9, MpLTPd10, MpLTPd11, and MpLTPd12. Type G nsLTPs include AtLTPg1, AtLTPg2, AtLTPg3, AtLTPg4, AtLTPg7, AtLTPg8, AtLTPg9, AtLTPg10, AtLTPg11, AtLTPg13, AtLTPg14, AtLTPg15, AtLTPg19, AtLTPg23, AtLTPg24, AtLTPg26, AtLTPg29, AtLTPg31, OsLTPg1, OsLTPg2, OsLTPg4, OsLTPg7, OsLTPg9, OsLTPg10, OsLTPg12, OsLTPg13, OsLTPg14, OsLTPg15, OsLTPg16, OsLTPg18, OsLTPg22, OsLTPg24, PpLTPg3, PpLTPg6, PpLTPg9, PpLTPg11, PpLTPg12, SmLTPg3, SmLTPg5, SmLTPg7, SmLTPg8, MpLTPg1, MpLTPg2, MpLTPg3, and MpLTPg4