Fig. 7

Functional complementation of the yeast ubc13 mms2 double mutant by BnUBC13s and AtUEV1D. WXY955 (ubc13Δ mms2Δ) cells co-transformed with vectors pGBT9E and pGAD424E or with plasmids containing AtUEV1D and indicated BnUBC13s were grown in the SD-Leu-Trp selective medium overnight. After adjustment with cell density, cells were printed onto YPD plates containing 0.025% MMS gradient and the plates were incubated at 30 ºC for 2 days before being photographed. The arrow points to increasing MMS concentrations. Several independent colonies from each transformation were examined with comparable results, and only one set of plates is shown here. Wild type, HK578-10D.