Fig. 6
Physical interactions between BnUbc13s and AtUev1D in a GST pulldown assay. Co-purified GST-AtUev1D and His6-BnUbc13s were added to microspin columns. After incubation and washing, the columns were eluted with reduced glutathione and subjected to SDS-PAGE analysis. A AtUev1D with BnUbc13A-D. B AtUev1D with BnUbc13E-H. Identities of key bands are marked. Unprocessed WB images shown in A, B are given in Supplementary Fig. S3A, B