Fig. 5

Physical interactions between BnUbc13s and AtUev1D in a yeast two-hybrid assay. PJ69-4 A cells co-transformed with vectors pGBT9E (Gal4_BD) and pGAD424E (Gal4_AD) or plasmids carrying AtUEV1D and indicated BnUBC13s were grown in the SD-Leu-Trp medium overnight. After adjustment of cell density, cells were replicated on SD-Trp-Leu (control), SD-Trp-Leu-His plus various concentrations of 3-AT, and SD-Trp-Leu-Ade, followed by incubation for 3 days at 30 ^oC before being photographed. Five independent colonies from each transformation were examined with comparable results, and only one set is shown here