Fig. 3

Functional complementation of the yeast ubc13 rev3 double mutant by BnUBC13s. WXY904 (ubc13Δ) and WXY921 (ubc13Δ rev3Δ) cells transformed with vector pGBT9E or plasmids carrying indicated BnUBC13 genes were grown in the selective medium overnight. After adjustment of the cell density, cells were printed onto plates containing different concentrations of MMS gradient, and the plates were incubated at 30 ºC for 2 days before being photographed. The arrow points to increasing MMS concentrations. Several independent colonies from each transformation were examined with comparable results, and only one set of plates is shown here. Wild type, HK578-10D; rev3Δ, WXY1233.