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Fig. 2 | BMC Plant Biology

Fig. 2

From: Systematic characterization of Brassica napus UBC13 genes involved in DNA-damage response and K63-linked polyubiquitination

Fig. 2

Functional complementation of the yeast ubc13 null mutant by BnUBC13s. WXY904 (ubc13∆) cells transformed with vector pGBT9E or plasmids carrying indicated BnUBC13 genes were grown in the selective medium overnight and the cell density was adjusted. A A serial dilution assay. Cells were diluted and printed onto YPD plates with or without different concentrations of 4NQO or MMS. For the UV irradiation, plates containing printed cells were exposed to 254 nm UV at given doses. B A gradient plate assay. Cells were printed to a square plate across the MMS gradient at given concentrations. The plates were incubated at 30 ºC for 2 days before being photographed. The arrow points to increasing MMS concentrations. Several doses of DNA-damaging agents were examined and only one representative plate is shown. For each sample, several independent colonies from each transformation were examined with comparable results, and only one set of plates is shown. Wild type, HK578-10D.

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