Fig. 1

Sequence analysis of Ubc13s from B. napus (Bn) and A. thaliana (At). A Predicted amino acid sequences of UBC13 gene products from B. napus and Arabidopsis were aligned by the BioEdit software version 7.2.5. Identical residues shared by the majority of Ubc13s are highlighted. Amino acid residues required for the interaction with UEV are marked by red asterisks, required for the interaction with a RING E3 is marked by a blue asterisk, and the active site Cys residue is marked by a green asterisk. B A phylogenetic tree based on B. napus and Arabidopsis Ubc13 family amino acid sequences was constructed by using MEGA7.0.26 and iTOL. Lines with different colors represent different branches: black, Arabidopsis; red and purple, two separate branches of B. napus. Yellow and green rectangles highlight Ubc13 proteins from B. napus and Arabidopsis, respectively. Purple circles indicate bootstrap levels as indicated