Fig. 5
The expression of heat shock protein genes in leaves of Z. xanthoxylum under different heat treatments. (a) and (b) show the number of DEGs at 40 °C and 45 °C for 0.5 h, respectively; (c) and (d) show the number of DEGs at 40 °C and 45 °C for 6 h, respectively. The red upward arrows and black downward arrows show the total number of up-regulated DEGs and down-regulated DEGs, respectively. Venn diagrams showing the number of unique and common DEGs related to sHSP (e), HSP70 (f) and HSP90 (g) in leaves of Z. xanthoxylum under heat treatments. (h) Heat maps of transcript abundance for 47 common genes of sHSP in Z. xanthoxylum at 40 °C and 45 °C for 0.5 h and 6 h. (i) Heat maps of transcript abundance for 12 common genes of HSP70 in Z. xanthoxylum at 40 °C and 45 °C for 0.5 h and 6 h. (j) Heat maps of transcript abundance for 19 common genes of HSP90 in Z. xanthoxylum at 40 °C and 45 °C for 0.5 h and 6 h. Gene expression levels were calculated and normalized using the FPKM method (h-j). (k) Venn diagrams showing the number of DEGs related to HSP90 in leaves of Z. xanthoxylum at 40 °C and 45 °C for 0.5 h. (l) Heat maps of transcript abundance for 30 up-regulated HSFs identified only at 40 °C for 0.5 h. The relative expression levels were calculated after log2 transformation of FPKM values plus 1