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Fig. 7 | BMC Plant Biology

Fig. 7

From: Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold

Fig. 7

Effect of exogenous 2,4-epibrassinolide (EBL), methane-rich water (MRW) and brassinazole (Brz) on the XTH and POD activity during AR development in marigold explants. The primary roots of 5-day-old seedlings were removed, and then the marigold explants were incubated with 1 μmol L− 1 EBL, 20% MRW, 0.5 μmol L− 1 Brz (a synthetic inhibitor of EBL) alone or together for 48 h, as indicated. The activities of xyloglucan endotransglucosylase/hydrolase (XTH, A), peroxidase (POD, B) were measured at 0, 6, 12, 24 and 48 h after treatment. Values (mean ± SE) are the means of three independent experiments (n = 200). Bars with different lowercase letters are significantly different (Duncan’s multiple range test; p < 0.05)

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