Fig. 6From: Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigoldEffect of exogenous 2,4-epibrassinolide (EBL), methane-enriched water (MRW) and brassinazole (Brz) on the CMCase, xylanase and laccase activity during AR development in marigold explants. The primary roots of 5-day-old seedlings were removed, and then the marigold explants were incubated with 1 μmol L− 1 EBL, 20% MRW, and 0.5 μmol L− 1 Brz (a synthetic inhibitor of EBL) alone or together for 48 h, as indicated. The activities of carboxymethyl cellulose (CMCase, A), xylanase (B), laccase (C) were measured at 0, 6, 12, 24 and 48 h after treatment. Values (mean ± SE) are the means of three independent experiments (n = 200). Bars with different lowercase letters are significantly different (Duncan’s multiple range test; p < 0.05)Back to article page