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Fig. 5 | BMC Plant Biology

Fig. 5

From: Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigold

Fig. 5

Effect of exogenous 2,4-epibrassinolide (EBL), methane-rich water (MRW), and brassinazole (Brz) on cellulose hemicellulose and lignin content during AR development in marigold explants. The primary roots of 5-day-old seedlings were removed, and then the marigold explants were incubated with 1 μmol L− 1 EBL, 20% MRW, 0.5 μmol L− 1 Brz (a synthetic inhibitor of EBL) alone or together for 48 h, as indicated. The contents of cellulose (A), hemicellulose (B), and lignin (C) were measured at 0, 6, 12, 24, and 48 h after treatment. Values (mean ± SE) are the means of three independent experiments (n = 500). Bars with different lowercase letters are significantly different (Duncan’s multiple range test; p < 0.05)

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