Fig. 4From: Brassinosteroids is involved in methane-induced adventitious root formation via inducing cell wall relaxation in marigoldEffect of exogenous methane-rich water (MRW) on the enzymatic activities of steroid 5α-reductase (DET2), 22α-hydroxylase (DWF4) and BR-6-oxidase (BR6ox) during AR development in marigold explants. The primary roots of 5-day-old seedlings were removed, and then the marigold explants were incubated with 20% MRW for 24 h. The activities of DET2 (A), DWF4 (B), and BR6ox (C) were measured at 0 and 24 h after treatment. Values (mean ± SE) are the means of three independent experiments (n = 30). ** indicates highly significant differences (independent T-test, p < 0.01)Back to article page