Fig. 3

Effect of exogenous methane-rich water (MRW) on the content of endogenous campestanol (CN), cathasterone (CT), castasterone (CS), and 2,4-epibrassinolide (EBL) during AR development in marigold explants. The primary roots of 5-day-old seedlings were removed, and then the marigold explants were incubated with 20% MRW for 24 h. The content of CN (A), CT (B), CS (C), and EBL (D) was measured at 0 and 24 h after treatment. Values (mean ± SE) are the means of three independent experiments (n = 30). * and ** indicate significant difference and highly significant difference (independent T-test, p < 0.05 or p < 0.01), respectively