Fig. 5

Disrupted dimerization of OsphyC in the lhd3. A Yeast two-hybrid assay of interactions between OsphyC and OsphyB (or OsphyC) from the WT and the lhd3. The interaction between D1 and TUD1 was used as a positive control, and empty pGBKT (bait) and pGADT (prey) were used as negative controls. QDO indicates SD/-Trp-Leu-His-Ade medium. B BiFC assay of the interaction between OsphyC and OsphyB (or OsphyC) from the WT and the lhd3 in N. benthamiana epidermal cells. C OsphyC protein levels at two selected time points (10 AM and 10 PM) in the WT and the lhd3 plants grown under natural LD conditions. Each lane was loaded equally, and followed by protein gel blot analyses using antibodies against OsphyC and OsActin as the loading control