Fig. 2

Map-based cloning and identification of the LHD3 gene. A Location of the LHD3 on the long arm of rice chromosome 3, and the gene was further mapped to a 46 kb interval between molecular markers C5 and C6. B The candidate region contained eight ORFs. The mutation site in the lhd3 was shown at position 1930 in LOC_Os03g54084 and change the serine to threonine at position 577. C Phenotypes of transgenic lines. Empty vector was used in the transgenic line as the negative control. Scale bar = 20 cm. D Days to heading comparison among the WT, lhd3 and transgenic plants in both Nanchang city, Jiangxi Province (NC.JX) and Sanya city, Hainan Province (SY. HN). Error bars indicate SD; n = 20 plants. * and ** represented the significant difference (P < 0.05) and extremely significant difference (P < 0.01), respectively