Fig. 1

Development of barcode oligo-FISH probes for So. bicolor and Sa. spontaneum chromosome identification. a Oligos were selected from a total of 20 chromosomal regions on 10 sorghum chromosomes (11 red regions and 9 green regions). All 10 basic chromosomes can be distinguished simultaneously based on number and location of the red/green signals. The red/green dots represent the color of the signals produced by the probe, and its position on the chromosome is drawn based on the data in Table S1. b FISH analysis of diploid So. bicolor BTx623 (2n = 2x = 20) using the barcode oligo probes on mitotic metaphase chromosomes. c FISH analysis of autotetraploid Sa. spontaneum Np-X (2n = 4x = 40) using the barcode oligo probes on mitotic metaphase chromosomes. The top panels show a complete metaphase cell from So. bicolor and Sa. spontaneum, respectively. The bottom panel shows the homologous chromosomes of each of the 10 chromosomes digitally excised from the same cell. The centromeres positions of the chromosomes are aligned by a white dotted line. Bars = 10 μm