Fig. 11
From: GhAAO2 was observed responding to NaHCO3 stress in cotton compared to AAO family genes
The phenotype of cotton leaves after gene silencing and expression analysis of GhAAO2 under NaHCO3 stress. PDS: positive control. CK: normal plant. pYL156: Blank control with pYL156 empty vector. pYL156: GhAAO2: silent plants. NaHCO3-CK: Normal plants after NaHCO3 treatment. NaHCO3-pYL156: Blank control with pYL156 empty vector after NaHCO3 treatment. NaHCO3-pYL156: GhAAO2: silent plants after NaHCO3 treatment. A: Phenotype of cotton leaves after gene silencing. B: Phenotype of cotton leaves in silenced plants under NaHCO3 stress. C: qRT-PCR for GhAAO2 under NaHCO3 stress. Note: CK-pYL156: Blank control with pYL156 empty vector; CK-GhAAO2: silent plants. NaHCO3-pYL156: Blank control with pYL156 empty vector after NaHCO3 treatment. NaHCO3-GhAAO2: silent plants after NaHCO3 treatment. D: MDA content of empty control and VIGS plants under normal growth and NaHCO3 stress. E: PRO content of empty control and VIGS plants under normal growth and NaHCO3 stress