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Fig. 4 | BMC Plant Biology

Fig. 4

From: A survey of the full-length transcriptome of Gracilariopsis lemaneiformis using single-molecule long-read sequencing

Fig. 4

Alternative polyadenylation analysis. A Distribution of the number of poly (A) sites per gene. B The statistics of APA locations within genes. C Validation of polyadenylation sites by RT–PCR. PCR was performed using a 3′ RACE reverse primer and gene-specific forward (F) primer. The colored arrows indicate the locations of APAs and point to the corresponding PCR product. Exons are represented by open boxes. Equal amounts of cDNA in the samples were verified using actin as an internal control

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