Fig. 3

PCR validation of AS events identified in SMRT-Seq data. cDNAs from control (CK), and treated (Cu²⁺ (Cu), Cu²⁺ combined with ACC (CA), 33 °C (HT) and 33 °C combined with ABA (HA)) samples were used for RT–PCR. Exons are represented by filled boxes, introns are represented by lines, and 3′ and 5′ UTRs are represented by open boxes. The annotated isoforms are shown in black. The novel isoforms from SMRT-Seq reads and sequencing of PCR products are colored and indicated by arrows. The right panel shows the predicted protein of each isoform and putative domains identified by MEME. The location of the predicted stop codon in the transcripts is represented by a vertical line. The gene ID is shown at the left in each panel. M, lane with DNA size markers. F, forward primer and R, reverse primer