Table 1 Ten candidate reference genes and primer sequences (forward and reverse 5’-3’) used in the study
Gene symbol | Gene description | GenBank accession | Forward/Reverse primer(5’-3’) | Primers Tm (℃) | Amplicon length (bp) | RT–qPCR Efficiency | Correlation Coefficient |
|---|---|---|---|---|---|---|---|
ACT7 | actin-7 | actin-7 | GGAATCCACGAGACCACCTACA | 61.2 | 219 | 100.71 | 0.998 |
GATTGATCCTCCGATCCAGACA | 61.1 | ||||||
ACT11 | actin-11 | ON855356 | CAGGCTGTTCTTTCGCTTTAC | 57.4 | 228 | 113.81 | 0.998 |
AATGTCCCTCACAATTTCACG | 57.4 | ||||||
TUB | β-tubulin | ON855357 | CAGAGAGGAGTATCCAGACCGTAT | 58.7 | 218 | 98.35 | 0.999 |
GGTGGTTAAGATCGCCAAATGTAG | 62.2 | ||||||
DIM1 | dimethyladenosine transferase | ON855358 | AAGAACAATTTCCGTCCTCC | 55.8 | 292 | 103.45 | 0.984 |
CACTACCACATTCCACCTCC | 54.7 | ||||||
TBCB | tubulin-folding cofactor B | ON855359 | TGGTATCTCAGAACCCATCAGC | 59.1 | 246 | 92.14 | 0.997 |
GTGGAGGACAGTCAAAGTAACG | 56.6 | ||||||
ERF3A | eukaryotic peptide chain release factor GTP-binding subunit ERF3A | ON855360 | CGCCACCACAAGAAACCAAA | 61.7 | 297 | 98.96 | 0.998 |
TCTCATCCTCCGAGTCAACA | 55.7 | ||||||
GAPDH | glyceraldehyde-3-phosphate dehydrogenase | ON855361 | CTGTTCACTCCATCACTGCCAC | 60.2 | 282 | 103.14 | 0.998 |
TCATTTTTCCCTCAGATGCCTC | 60.8 | ||||||
EF-1α | elongation factor 1-α | ON855362 | CCTGGACATCGTGACTTTATC | 54.5 | 277 | 106.38 | 0.996 |
GGTTGTATCCGACCTTCTTTAG | 55.5 | ||||||
R3H | R3H domain-containing protein | ON855363 | TCGTCTAACCATTCTGCGGA | 59 | 178 | 102.78 | 0.995 |
CCCAAGCCCTTCTAAACCAC | 58.8 | ||||||
His | Histone | ON855364 | CCTCACCGTTACCGCCCT | 59.46 | 182 | 89.67 | 0.999 |
GCCTCAGCTGCCTCTTGC | 59.46 | ||||||
P5CS | Δ1-Pyrroline-5-carboxylate synthetase | ON855365 | TGCCCTTTGGGTGTCCTCTT | 61.7 | 132 | 94.32 | 1.000 |
TGCATTTGATCGCTTAGCTTCT | 59.8 |