Fig. 3

Transcriptional levels of the hydrogen peroxide detoxifying enzymes after B. japonicum inoculation and salt treatment. Plants were inoculated with B. japonicum and after 7 days they were treated with 100 mM NaCl for 6 h before sample collection. qRT-PCR analysis of ROS scavenging enzymes from non-inoculated and B. japonicum-inoculated root tissue, with and without 100 mM NaCl treatment in (A) shoot and (B) root tissues. Graphs show expressions of Dehydroascorbate Reductase 1 (DHAR1), Catalase 3 (CAT3), Monodehydroascorbate Reductase 2 (MDAR2), Ascorbate Peroxidase 1 (APX1) and Manganese Superoxide Dismutase (MSD1) relative to the IPP2 reference gene ± standard error, with different letters representing significant differences separately for each gene, ANOVA, Tukey, p < 0.05