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Fig. 7 | BMC Plant Biology

Fig. 7

From: An uncharacterized protein NY1 targets EAT1 to regulate anther tapetum development in polyploid rice

Fig. 7

Phenotypic comparison and developing rice anther between H1 and ny1-related DEG mutant eat1. a Gene structure and CRISPR/Cas9 targets of EAT1. White areas, black areas, grey lines indicate untranslated regions (UTR), exons and introns, respectively. “ + ”, “-” indicated forward chain and reverse chain, respectively, where CRISPR/Cas9 target is located. The underline bases represent PAM (protospacer adjacent motif) for CRISPR/Cas9. b The plant type of H1 (WT) and eat1. Bars = 20 cm. c Mature panicles of H1 and eat1. Bars = 1 cm. d Floral organs of H1 and eat1. Bars = 0.5 mm. e I2-KI staining of H1 and eat1 pollen grains at mature stage. Green bars = 50 μm. (f-m) Anther transverse section analysis of eat1 in S6 f, S7 g, S8a h, i, S8b j, S9 k, S10 l, S11 m. Bars = 20 μm

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