Fig. 4

Anthocyanin accumulation and quantification in diploid embryos at 15 DAP derived from B73×CAU5 after culturing for 24 h under dark and light conditions. A–B Coloration of diploid embryos after culturing for 24 h under dark or light conditions. C Anthocyanin accumulation in diploid embryos grown in dark and light environments. D Anthocyanin quantification in diploid embryos grown under dark and light conditions at 0 and 24 h. Scale bars, 2 mm (A–B). ** represents p < 0.01 in the comparison of total anthocyanin content between dark and light conditions (two-tailed t test)