Fig. 3From: Accumulation of DNA damage alters microRNA gene transcription in Arabidopsis thalianaMIR transcription is inhibited by MMS treatment in zdp/ape2 mutants. A RT‒qPCR detection of the relative expression levels of pri-miRNAs in Col-0 and zdp-1/ape2-2 mutant plants treated with 0 ppm or 10 ppm MMS. EF1α was used as the internal control. Error bars represent the standard deviation calculated on the basis of 3 independent replicates. Statistically significant differences between different genotypes are indicated by different lower-case letters (P < 0.05, one-way ANOVA per gene, performed separately). B RT‒qPCR detection of the accumulation of miRNAs in Col-0 and zdp-1/ape2-2 plants treated with 0 ppm or 10 ppm MMS. U6 was used as the internal control. Error bars represent the standard deviation calculated from 3 independent replicates. Statistically significant differences between different genotypes are indicated by different lower-case letters (P < 0.05, one-way ANOVA per gene, performed separately)Back to article page