Fig. 1
From: Accumulation of DNA damage alters microRNA gene transcription in Arabidopsis thaliana
The simultaneous mutation of ZDP and APE2 leads to genome-wide alteration of miRNAs. A The relative frequency analysis of the 5′-terminal nucleotide of miRNAs in Col-0 plants and zdp-1/ape2-2 mutant plants. B Mean abundance analysis in zdp-1/ape2-2 mutant compared with Col-0 plants. The blue, yellow and pale colours represent downregulated, upregulated and unchanged miRNAs in zdp-1/ape2-2 mutant compared to Col-0 plants, respectively. C Northern blots showing the accumulation of miRNAs. U6 was the RNA loading control. RA indicates the relative fold change of miRNAs in zdp-1/ape2-2 and zdp-1/ape2-3 mutant compared with those in Col-0 plants. D Relative transcript levels of miRNA targets in Col-0, zdp-1/ape2-2, and zdp-1/ape2-3 plants. EF1α was used as the internal control. Statistically significant differences between different genotypes are indicated by different lower-case letters (P < 0.05, one-way ANOVA per gene, performed separately)