Skip to main content

Table 2 Compositions of the different plant tissue culture media used in this study

From: In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants

Component

MS [23]

B5[23]

WPM[23]

NM[23]

LS [25]

QL [26]

DJ[27]

CHU[28]

Ammonium nitrate

1650.0

-

400.0

720.0

1650.0

400

-

-

Ammonium sulphate

-

134.0

-

-

-

-

400.0

463.0

Boric acid

6.2

3.0

6.2

10.0

6.2

6.2

10.62

1.6

Calcium nitrate

-

-

386.0

-

-

-

-

-

Calcium chloride.2H2O

-

150.0

-

-

440.0

-

300.0

-

Calcium chloride, anhydrous

332.2

-

72.5

-

-

1200.0

-

125.34

Cobalt chloride•6H2O

0.025

0.025

-

-

0.025

0.025

0.0025

-

Cupric sulphate•5H2O

0.025

0.025

0.25

0.025

0.025

0.025

0.0025

-

Na2-EDTA

37.26

37.3

37.3

37.25

37.26

-

37.30

37.3

Sodium phosphate monobasic

-

130.42

-

-

-

-

250.0

-

Ferrous sulphate•7H2O

27.8

27.8

27.85

27.85

27.86

-

27.8

27.8

Magnesium sulphate

180.7

122.09

180.7

90.34

370.0

360.0

244.17

90.37

Manganese sulphate•H2O

16.9

10.0

22.3

18.94

22.3

1.0

1.68

3.33

Molybdic acid, sodium salt, 2H2O

0.25

0.25

0.25

0.25

0.25

0.25

0.0025

-

Potassium iodide

0.83

0.75

-

-

0.83

0.08

1.58

0.80

Potassium nitrate

1900.0

2500.0

-

950.0

1900.0

1800.0

2000.0

2830.0

Potassium sulphate

-

-

990.0

-

-

-

-

-

Potassium chloride

-

-

-

-

-

-

600.0

-

Potassium phosphate monobasic

170.0

-

170.0

68.0

170.0

270.0

-

400.0

Zinc sulphate•7H2O

8.6

2.0

8.6

10.0

8.6

8.6

1.06

1.5

Myo-inositol

100.0

100.0

100.0

100.0

100.0

-

100.0

-

Nicotinic acid

1.0

1.0

0.5

5.0

-

-

1.0

0.5

Pyridoxine HCl

1.0

1.0

0.5

0.50

-

-

1.0

0.5

Folic acid

-

-

-

0.50

-

-

-

-

Thiamine HCl

10.0

10.0

1.0

0.50

0.4

-

10.0

1.0

Glycine

-

-

2.0

2.0

-

-

-

2.0

Biotin

-

-

-

0.05

-

-

-

-

  1. Every ingredient in mg·L−1