Table 1 The main results of micromorphological, histochemical and ultrastructural features of flowers of Brasiliorchis schunkeana, and chemical composition of lips
Floral part and surface/method | Micromorphology (SEM) | Histochemistry (TBO, ABB, SBB, PAS, FeCl3, Ruthenium Red) | Ultrastructure (TEM) | Chemical composition (GC/MS) |
|---|---|---|---|---|
adaxial (inner) surface | ||||
 lip base | glabrous surface | huge amounts of lipids (SBB), small amounts of proteins (ABB), few, tiny strach grains (PAS), dihydroxyphenols (FeCl3) | a few residues of secreted materials, cuticle sometimes ruptured, lipoid and phenolic nature of secretion, lipids in cytoplasm,phenols in vacuoles, periplasmic spaces with vesicles and flocculent material, globules with osmiophilic material, vacuole with large vesicles with osmiophilic borders, | 16 compounds in methanolic extract (i.a. including fatty acid methyl esters, monoacylglycerols, dicarboxylic acids, glycerol, trehalose), 6 compounds in dichloromethane extract (i.a. phenols) |
 lip callus | glabrous surface, a few traces of secretion | idioblasts with raphides (TBO, PAS) | remnants of secretion, phenolic accumulation in vacuoles, | |
 lip apex | conical to villiform papillae, a few traces of secretion | idioblasts with raphides (TBO, PAS) | papillae with osmiophilic phenolic content, vacuoles with osmiophilic phenolic globules, and phenolic content, vacuolar fragmentation | |
 lip margins | conical to villiform papillae |  |  | |
abaxial (outer) surface | ||||
 lip | conical papillae |  |  |  |
 dorsal sepal |  | adaxial (inner) surface with papillae and few sessile trichomes (TBO, PAS), idioblasts with raphides (TBO, PAS), lipids (SBB), dihydroxyphenols (FeCl3) |  |  |
 lateral sepals |  | small amounts of lipids (SBB), few tiny starch grains (PAS), |  |  |
 petals |  | lipids (SBB) |  |  |
 column foot |  | dihydroxyphenols (FeCl3) |  |  |