Fig. 5
From: ThASR3 confers salt and osmotic stress tolerances in transgenic Tamarix and Arabidopsis
Analysis of ROS accumulation and antioxidant enzyme activities in three types (VC, OE and IE) transgenic T. hispida plants under NaCl or mannitol treatment. A 3,3′-Diaminobenzidine (DAB) and B Nitroblue tetrazolium (NBT) staining were performed to detect H2O2 and O2·− accumulation in young branches of T. hispida plants. Analysis of H2O2 C and MDA D contents, SOD E and POD F activities in three types (VC, OE and IE) transgenic T. hispida plantlets. 15 plants were selected for photography in each treatment, and the most representative photos were selected and combined. The asterisks (*) indicate a significant difference (P < 0.05) between transformed (OE and IE) and control (VC) plants