Fig. 4

Characterization of wild type (WT) and transgenic RNAi_SlS5H (lines 14 and 16) tomato plants infected with Pseudomonas syringae pv. tomato DC3000 (Pst). Tomato plants were inoculated with Pst by immersion. Leaf samples were taken 24 hours after bacterial infection and analyzed for bacterial growth (A), S5H (B) and PR1 (C) gene expression, and accumulation of phenolic compounds (Salicylic Acid, SA, D; Gentisic Acid, GA, E). MOCK represents the non-inoculated plants. The qRT-PCR values were normalized with the level of expression of the actin gene. The data are presented as means ± standard deviation of a representative experiment (n > 3). Statistically significant differences (p-value < 0.05) between genotypes and infected or mock-treated plants are represented by different letters. Significant differences in SA and GA accumulation are analysed in free (x) and total (x’) phenolic compounds