Fig. 4

Identification of Cscpftsy for yellow leaf phenotype. a Initial mapping with 245 F₂ individuals placed Cscpftsy at 0.9 Mb physical interval flanked by yb-Indel3-2 and yb-Indel3-3 in chromosome 3. b Further mapping with 737 F₂ individuals narrowed Cscpftsy down a 100 Kb physical interval flanked by AInd3-16 and AInd3-24, c dCAPS3-1 was developed based the candidate SNP to identify the co-segregation among five recombinants. d 20 candidate genes in the fine mapping region. e The structure of candidate gene CsaV3_009150 and candidate SNP(G-A), black boxes mean exons and lines mean introns. f The candidate SNP changed Glycine (Gly) to Arginine (Arg)