Fig. 2

The leaf blade of R. pseudoacacia besides a gall (control). a Cross section through a young fresh leaf blade in their side part, with small vascular bundles; cryostate section, stained with Ehrlich hematoksylin. b Cross section through the same, young fresh leaf with palisade and sponge mesophyll and a bigger (no central) vascular band; cryostate section; stained with Ehrlich hematoksylin. c Transverse, free hand section of older, mature fresh leaf of O. robiniae, no stained, with a mesophyll (palisade and spongy), vascular bundles enclosed by bundle sheaths consisting of almost colourless parenchyma (stars). Bundle sheaths extensions extend from the veins to the upper and lower epidermis. The layer of parenchyma with small amount of chloroplasts extends under lower epidermis. d Part of the whole cleared leaf from the side of abaxial epidermis; many mechanical trichomes and reticulate venation are visible; slide stained with Trypane blue. Abbreviations: eb - abaxial (lower) epidermis, ed. - adaxial (upper) epidermis, mp - palisade mesophyll, ms - spongy mesophyll, vb - vascular bundle, t - trichome, bs - bundle sheath, * colourless parenchyma. Bar = 20 μM