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Fig. 7 | BMC Plant Biology

Fig. 7

From: Cellular messengers involved in the inhibition of the Arabidopsis primary root growth by bacterial quorum-sensing signal N-decanoyl-L-homoserine lactone

Fig. 7

Up-regulated cytosolic Ca2+ induced ROS and NO reaction which is necessary for C10-HSL induced root growth inhibition. (a) Relative primary root growth of Col-0 seedlings exposed to 30 µM C10-HSL with or without 100 µM LiCl plus 300 µM LaCl3 compared with control for 5 d, bar = 1 cm, n = 40. (b) Detection of cytosolic Ca2+ concentration in the roots of Col-0 seedlings exposed to 30 µM C10-HSL with or without 100 µM LiCl plus LaCl3 compared with untreated seedlings using the Ca2+ specific florescent probe Fluo4-am and quantification of Ca2+-specific fluorescence intensities, bar = 100 μm, n = 30. (c) Detection of ROS production in the roots of Col-0 seedlings exposed to 30 µM C10-HSL with or without 100 µM LiCl plus LaCl3 for 15 min using the specific florescent probe DCF-DA and quantification of specific fluorescence intensities, bar = 100 μm, n = 30. (d) Detection of NO production in the roots of Col-0 seedlings exposed to 30 µM C10-HSL with or without 100 µM LiCl plus LaCl3 for 15 min using the NO specific florescent probe DAF-2DA and quantification of NO-specific fluorescence intensities, bar = 100 μm, n = 30. Control or Con refers to solvent control. All the error bars represent +/- SD. (Different letters indicate significantly different values, P < 0.05 by Tukey’s test)

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