Fig. 6

C10-HSL improved cytosolic Ca²⁺ concentration in wild-type seedlings. (a) Changes in [Ca²⁺]_cyt in apoaequorin-transformed Arabidopsis root cells with 10 nM, 100 nM, 1 µM, 10 µM, 30 µM C10-HSL. (b) Changes in [Ca²⁺]_cyt in Arabidopsis root cells were measured upon addition of 30 µM C10-HSL after pretreatment with LaCl₃, EGTA and Verapamil respectively. (c, d) Detection of cytosolic Ca²⁺ concentration in the roots of Col-0 seedlings exposed to 0 nM, 10 nM, 100 nM, 1µM, 10 µM and 30 µM C10-HSL using the Ca²⁺ specific florescent probe Fluo4-am, bar = 100 μm. Control or Con refers to solvent control. All the error bars represent +/- SD. (Different letters indicate significantly different values, P < 0.05 by Tukey’s test)