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Fig. 3 | BMC Plant Biology

Fig. 3

From: Cellular messengers involved in the inhibition of the Arabidopsis primary root growth by bacterial quorum-sensing signal N-decanoyl-L-homoserine lactone

Fig. 3

ROS is involved in C10-HSL-mediated inhibition of primary root growth. (a, b) Detection of ROS level in the roots of wild-type seedlings exposed to 30 µM C10-HSL for 0–60 min using the ROS-specific florescent probe DCF-DA (a), and quantification of ROS-specific fluorescence intensities (b) bar = 50 μm. (c, d) Detection of ROS level in the roots of wild-type seedlings exposed to 30 µM C10-HSL with or without 500 µM CAT for 15 min using the ROS-specific florescent probe DCF-DA (c), and quantification of ROS-specific fluorescence intensities (d), n = 30, bar = 50 μm. (e, f) Relative primary root growth of wild-type seedlings exposed to 30 µM C10-HSL with or without 1.5 mM H2O2 and 500 µM CAT for 5 d, bar = 1 cm, n = 40. (g) Relative primary root growth of Col-0 and noa1 seedlings exposed to 30 µM C10-HSL for 5 d, n = 40. Control or Con refers to solvent control. All the error bars represent +/- SD. (Different letters indicate significantly different values, P < 0.05 by Tukey’s test. *P < 0.05, **P < 0.001, Student’s t test)

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