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Fig. 8 | BMC Plant Biology

Fig. 8

From: Physiological and protein profiling analysis provides insight into the underlying molecular mechanism of potato tuber development regulated by jasmonic acid in vitro

Fig. 8

Immunoblot analysis of key proteins in JA biosynthesis and regulatory pathway during tuber development. The immunoblot expression and iTRAQ expression of COI1 (A), HSP90 (B), and LOX2 (C) were analyzed under different JA concentration. Data are presented as means ± SE for three independent experiments. In order to save antibody and chromogenic reagent, the membranes were cut into strips using the molecular weight standard as a guide after transfer to a PVDF membrane. The first membrane was cut just 1 cm above and below the 70 KDa molecular weight marker and used for COI1 immunoblot. The second membrane was cut just between the 70 KDa and 100 KDa molecular weight markers and used for HSP90 immunoblot. The third membrane was cut just 1 cm above and below the 100 KDa molecular weight marker and used for LOX2 immunoblot. The original gels and blot strips are presented in Additional files 5, 6, 7 and 8: Fig. S1-4

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