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Fig. 7 | BMC Plant Biology

Fig. 7

From: The diurnal emission of floral scent in Oncidium hybrid orchid is controlled by CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) through the direct regulation on terpene synthase

Fig. 7

Transactivation assays showing CCA1 regulation on TPS promoter activity by binding on CBS motif a The schematic model of effector and reporter constructs. OncSBCCA1 was driven by pCaMV35S promoter and pOncSBTPS was used to drive Firefly Luciferase. As a negative control, CBS (-AGATTTTT-) on the pOncSBTPS_mCBS was replaced as -GGGTTTTT-, in order to prevent the interactions with OncSBCCA1 transcription factor. b The promoter activities assay with or without OncSBCCA1 transcription factor. Firefly Luciferase activities were detected to represent the interactions between CCA1 and CBS. The data suggested that OncSBCCA1 upregulates the gene which was driven by the promoter containing CBS (n = 7, P < 0.05: *, P < 0.01: **)

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BMC Plant Biology

ISSN: 1471-2229

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