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Fig. 1 | BMC Plant Biology

Fig. 1

From: Comparative analysis of stress-induced calcium signals in the crop species barley and the model plant Arabidopsis thaliana

Fig. 1

Selection and characterization of Hv-AEQcyt lines. A Ca2+-dependent photon release in relative light units (RLU) of different transgenic barley lines expressing the recombinant APOAEQUORIN (Hv-AEQcyt #8, #13, #17, and #18) after discharge of aequorin. B [Ca2+]cyt of Hv-AEQcyt #17 and #18 in response to H2O2 (10 mM). The dashed line represents the time point of H2O2 injection. C Transcript level of APOAEQUORIN in leaves and roots of Hv-AEQcyt #18 determined against a cDNA dilution series and normalized to Actin (AY145451.1). D Abundance of aequorin in leaves and roots of Hv-AEQcyt #18 and wild type (WT) as negative control determined by immunodetection using a specific antibody against aequorin. Values represent means ± SE of three independent replicates

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