Fig. 2

Transverse anther sections of three ginger species at different developmental stages stained with Periodic acid—Schiff (PAS, a staining method used to detect polysaccharides) and Sudan Black B (a staining method used to detect lipid), showing the distribution of polysaccharides and lipids in different anther tissues. Polysaccharides stained red, lipid stained black dots. A to D, Caulokaempferia coenobialis. E to H, Hornstedtia hainanensis. I to L, Zingiber nudicarpum. MMC, microspore mother cell; MSP, microspores; P, pollen grain; T, tapetum; TET, tetrad; the arrow shows the liquid in the locule. Scale bars, 50 μm