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Fig. 5 | BMC Plant Biology

Fig. 5

From: PhUGT78A22, a novel glycosyltransferase in Paeonia ‘He Xie’, can catalyze the transfer of glucose to glucosylated anthocyanins during petal blotch formation

Fig. 5

Structural models of PhUGT78A22 docked with UDP-glucose and sugar acceptors and analyses of PhUGT78A22 enzymatic reactions. A-D Structural models of PhUGT78A22 docked with UDP-glucose and substrate Cy (A), Cy3G (B), Pn (C) and Pn3G (D). The structural model shows the surface binding mode (left) and key amino acid residues for the sugar donor and acceptor positions (right). The substrates are colored yellow and the UDP-glucose is colored green. The surrounding residues in the binding pocket were colored in purple sticks, the backbone of the receptor is depicted as purple cartoon, the hydrogen bonds are depicted as yellow dashed lines, and the salt bridges are depicted as magenta dashed lines. (E & F) Representative UPLC-DAD chromatograms of GST-PhUGT78A22 with Cy3G (E) or Pn3G (F) as substrate (left). Reactions with GST proteins were used as negative controls (right)

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BMC Plant Biology

ISSN: 1471-2229

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