Fig. 5

Effects of ACC treatment on GFP-tagged Vd991 colonization process of YZ1 roots. A GFP fluorescence intensities of confocal scanning of YZ1 roots with or without the 100-μM ACC treatment at 12, 24, 48 and 72 h after inoculation with GFP-tagged Vd991. The white dotted line shows the local enlarged drawing. Bar = 20 μm. B Statistics of the fluorescence intensity in (A). C Relative V. dahliae biomasses in the infected roots as assessed by qRT-PCR. GhUBQ7 was used as the internal control to normalize all the data. Experiments were repeated three times with similar results. Values are means ± SDs (Student’s t-test; *P < 0.05, **P < 0.01)