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Table 6 Primer sequence, band length, total amplified bands, monomorphic, polymorphic, unique bands and percentage of polymorphism and efficiency of ISSR and SRAP analysis of three A. maurorum genotypes

From: Eco-biochemical responses, phytoremediation potential and molecular genetic analysis of Alhagi maurorum grown in metal-contaminated soils

Primer Name

Primer sequence (5`----3`)

Band length (bp)

TAB

NMB

NPB

NUB

PPB

MBF

PIC

RP

ISSR markers

 49A

CAC ACA CAC ACA AG

215–385

2

1

1

–

50%

0.83

0.22

0.67

 HB-9

CAC CAC CAC GC

380–435

2

2

–

–

–

1.00

0.00

0.00

 HB-10

GAG AGA GAG AGA CC

280–470

2

1

1

–

50%

0.83

0.22

0.67

 HB-11

GTG TGT GTG TGT TGT CC

460–810

2

1

1

–

50%

0.83

0.22

0.67

 HB-13

GAG GAG GAG C

470–975

3

2

1

–

33.33%

0.89

0.15

0.67

Total

–

11

7

4

–

–

–

–

–

Average

200–900

2.2

1.4

0.8

–

36.36%

0.88

–

0.53

DI of all ISSR primers = 0.16

SRAP markers

 ME1xEM7

F ME-1: GAGTCCAAACCGGATA

R EM-7: GACTGCGTACGAATTCAA

275–1500

6

–

6

3

100%

0.50

0.45

4.02

 ME1xEM8

ME-1: GAGTCCAAACCGGATA

EM-8: CTGCGTACGAATTCAC

320–680

4

2

2

–

50%

0.83

0.22

1.36

 ME2xEM6

ME-2: TGA GTC CAA ACC GGA GC

EM-6: GAC TGC GTA CGA ATT CC

145–845

7

2

5

2

71.42%

0.66

0.32

3.36

 ME2xEM7

ME-2: TGA GTC CAA ACC GGA GC

Em-7: GACTGCGTACGAATTCAA

135–185

3

2

1

1

33.33%

0.78

0.15

0.66

 ME5xEM6

Me-5: TGAGTCCAAACCGGAAG

EM-6: GAC TGC GTA CGA ATT CC

110–200

3

1

2

1

66.66%

0.66

0.30

1.34

 ME5xEM8

Me-5: TGAGTCCAAACCGGAAG

EM-8: CTGCGTACGAATTCAC

100–250

2

2

–

–

–

1.00

0

0

Total

–

25

9

16

7

–

–

–

–

Average

100–1500

4.17

1.5

2.67

1.17

64%

0.74

–

1.79

DI of all SRAP primers = 0.24

Combined ISSR and SRAP

 

36

16

20

7

55.55%

–

–

–

  1. TAB Total amplified bands, NMB Number of monomorphic bands, NPB Number of polymorphic bands; NUB Number of unique bands, PPB Percentage of polymorphic bands, MBF Mean of band frequency, PIC polymorphism information content, Rp Resolving power and DI Diversity index