Fig. 7

qRT-PCR assay of some key genes identified in the network. A dual y-axis plot illustrating in parallel comparisons of each individual gene examined detected by RNA-seq (expression fold change compared to 0 h of TT, blue) and qRT-PCR (relative expression levels, red). The right coordinate (y axis) with blue represents the expression fold change of RNA-seq, the left coordinate (y axis) with red represents the relative expression levels of qRT-PCR. The transcription levels of each gene in each time point were normalized relative to the internal control (NtActin7). Relative expression levels of genes examined were calculated and expressed as 2^-ΔΔCT [63] relative to the expression levels of the corresponding NtActin7, which were set as 1.0. The mean expression levels were calculated from three biological replicates. Error bars are standard deviations of three biological replicates. Significance test was determined using Student’s t test. “ns”: not significant, “*”: p < 0.05, “**”: p < 0.01, “***”: p < 0.001