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Table 8 The effect of Chlorella vulgaris crude extracts (Grown on BG11 and 100% TSW) with optimum conc. of acetic acid (IAA) and benzyl adenine (BA) (obtained from dose response curve) on Tomato plant

From: Enhancement of plant growth regulators production from microalgae cultivated in treated sewage wastewater (TSW)

Parameter

Shoot Length (cm)

Leaves, numbers

Root initiation

Branching

Leaf expansion

Control (MS medium)

5.217 ± 0.469bcd

3.500 ± 0.674 ab

 +  +  + 

-

-

DMSO

5.083 ± 0.925 c d

3.417 ± 0.669ab

 +  +  + 

-

-

IAA ( 0.5 ppm)

4.850 ± 0.454cde

3.083 ± 0.900bc

 +  +  + 

 +  +  + 

-

-

IAA (1.8 ppm)

6.850 ± 1.588a

3.583 ± 0.515ab

 +  +  + 

 +  +  + 

-

-

IAA (2.5 ppm)

6.008 ± 0.734 ab

3.750 ± 0.452a b

 +  +  + 

-

-

BA (10 ppm)

4.533 ± 0.624c de

2.833 ± 0.577c d

 + 

-

 + 

BA (25 ppm)

4.442 ± 0.516c d e

3.500 ± 0.674a b c

 + 

 +  + 

 + 

BA (50 ppm)

4.425 ± 0.636de

3.333 ± 0.651a b c

-

 +  +  + 

 +  +  + 

IAA + BA (0.5 + 10) ppm

4.008 ± 0.478e

3.167 ± 0.577abc

 +  +  + 

-

-

IAA + BA (1.8 + 25) ppm

4.533 ± 0.414de

3.667 ± 0.492ab

 +  +  + 

 + 

-

IAA + BA (2.5 + 50) ppm

4.525 ± 0.398 de

2.500 ± 0.674c

 + 

-

-

C. vulgaris IAA optim. conc.(BG-11)

5.650 ± 0.678b c

2.917 ± 0.996 bc

 +  +  + 

 +  +  + 

-

-

C. vulgaris BA optim. conc. (BG-11)

4.808 ± 0.543b cd

3.000 ± 0.603bcd

 +  +  +  + 

-

-

C. vulgaris(100%TSW) IAA optim. conc

5.050 ± 0.585 cd

3.750 ± 0.965a b

 +  +  +  +  + 

-

-

C. vulgaris (100%TSW) BA optim. conc

5.5750 ± 0.3306a

2.917 ± 0.515c d

 +  + 

-

-

  1. TSW treated sewage wastewater (-): Absence (6 +): the highest (5 +):high (4 +):moderate (3 +):low ( +):The lowest DMSO Dimethyl sulfoxide, MS Murashige and Skoog medium
  2. The values are the mean of three replicates ± SD
  3. Values followed by different letters are significantly different (p ≤ 0.05)