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Table 6 The effect of Anabaena oryzae crude extracts (Grown on BG110 and 100% TSW) with optimum conc. of acetic acid (IAA) and Benzyl adenine (BA) (obtained from dose response curve) on Tomato plant

From: Enhancement of plant growth regulators production from microalgae cultivated in treated sewage wastewater (TSW)

Parameter

Shoot Length (cm)

Leaves, numbers

Root initiation

Branching

Leaf expansion

Control (MS medium)

5.217 ± 0.469bcd

3.500 ± 0.674a

 +  +  + 

-

-

DMSO

5.083 ± 0.925 cd

3.417 ± 0.669a

 +  +  + 

-

-

IAA ( 0.5 ppm)

4.850 ± 0.454cde

3.083 ± 0.900ab

 +  +  + 

 +  +  + 

-

-

IAA (1.8 ppm)

6.850 ± 1.588a

3.583 ± 0.515 a

 +  +  + 

 +  +  + 

-

-

IAA (2.5 ppm)

6.008 ± 0.734 a b

3.750 ± 0.452 a

 +  +  + 

-

-

BA ( 10 ppm)

4.533 ± 0.624bc

2.833 ± 0.577 bc

 + 

-

 + 

BA (25 ppm)

4.442 ± 0.516b c d

3.500 ± 0.674 ab

 + 

 +  + 

 + 

BA (50 ppm)

4.425 ± 0.636b c d

3.333 ± 0.651a b

-

 +  +  + 

 +  +  + 

IAA + BA (0.5 + 10) ppm

4.008 ± 0.478 e

3.167 ± 0.577ab

 +  +  + 

-

-

IAA + BA (1.8 + 25) ppm

4.533 ± 0.414 d e

3.667 ± 0.492 a

 +  +  +  + 

 + 

-

IAA + BA (2.5 + 50) ppm

4.525 ± 0.398 d e

2.500 ± 0.674 b

 + 

-

-

A. oryzae IAA optim. Conc. ( BG-110)

5.208 ± 0.348bcd

2.583 ± 0.515 b

 +  +  + 

-

-

A. oryzae BA optim conc.(BG110)

4.8833 ± 0.3433b

2.500 ± 0.522c

 +  + 

-

-

A. oryzae(100% TSW) IAA optim. conc

5.708 ± 1.053bc

3.750 ± 0.452 a

 +  +  +  +  + 

-

-

A. oryzae(100%TSW) BA optim. conc

4.767 ± 0.543b

3.083 ± 0.669abc

 +  +  + 

-

-

  1. TSW Treated sewage wastewater (-):Absence (6 +): the highest (5 +):high (4 +):moderate (3 +):low ( +):The lowest DMSO: Dimethyl sulfoxide MS: Murashige and Skoog medium
  2. The values are the mean of three replicates ± SD
  3. Values followed by different letters are significantly different (p ≤ 0.05)