Fig. 7
From: Construction of drought stress regulation networks in potato based on SMRT and RNA sequencing data
Expression profiles and functional analysis of alternative splicing (AS) events. a. Classification of AS events. Diagrams show the following AS events: retained intron (RI), alternative 3′ splicing sites (A3), alternative 5′ splicing sites (A5), mutually exclusive exons (MX) and exon skipping (ES). The numbers of AS events. Filled boxes represent exons, introns are represented by black lines. b. The number of differentially expressed AS events in DCK, D1h, D3h, D6h, D12h, D24h and D48h. c. Reverse-transcription (RT)-PCR validation of AS events. The RT-PCR validation of AS events for four genes, all of them are intron retention. Gel bands in each figure show DNA markers and PCR results in five samples (DCK, D3h, D6h, D12h and D24h). The transcript structure of each isoform is shown in the right panel. Exons are represented by blue filled boxes, introns are represented by lines, and the dotted line indicates that there is no intron corresponding to the first isoform in the second isoform of the same gene. Primers are designed to span the splicing events. PCR primers (F, forward and R, reverse) are shown on the first isoform of each gene. The length of each expected PCR product is shown after the transcript structure. d. WGCNA analysis of genetic modules relating to each sample. e. Enriched KEGG pathway of AS events involved in each module