Fig. 1

Sample preparation for RNA-seq analysis. Internodal segments (5 mm in length) were cut and divided into apical and basal regions before or after 1 week of culture on hormone-free B5 medium. 0a, apical region without culture; 0b, basal region without culture; 1a, apical region after 1 week of culture; 1b, basal region after 1 week of culture. n = 12 in 0a and 0b and 8 in 1a and 1b. Bar, 5 mm